Chromatography ColumnSKU: LS-3CC-GLR
Chromatography columns are pieces of glassware that help you separate chemical compounds. These borosilicate glass columns keep the stationary phase contained within while letting the mobile phase pass through. They are used in liquid and gas chromatography.
All our chromatography columns are made in the USA. For a complete guide to column chromatography, see our blog post.
80mm OD, 45/50 Rodaviss Joint, GL-18 Fittings
Uses of column chromatography:
- Removing pesticides,
- Separating constituents,
- Purifying individual chemical compounds.
We’ve experimented with many existing solutions on the market, but in the end, we decided that none of them were exactly what we were looking for.
So we created our own chromatography column that we could rely on in our own labs.
- Fritted disc
- GL-18 fitting
- PTFE stopcock
- Rodaviss top outer joint 45/50
- 3 inch (80mm) diameter x 24-inch effective length
- American-made, borosilicate glass.
A medium micron-fritted disc, made of fine porous glass. Liquids or gasses can pass through. The fritted discs are made by heating or smashing certain-sized glass particles together in a process known as sintering.
The bottom GL-18 connection of the chromatography column allows for quick draining via a tube or the GL-18 cap and barb may also be removed if the user does not require it.
For quick and easy flow control over your column.
Rodaviss Stopper with 45/50 top outer joint.
Now your glassware won’t get stuck, thanks to the black plastic ring below the glass protrusion on the dome. When the stopper is unscrewed, the ring presses up against the protruding glass and applies even pressure, allowing a safe disconnect of the joint every time.
Important column chromatography terms:
This phase is also called an eluent, and it can be a mixture of solvents or simply a pure solvent. As a general rule, you should optimize your solvents to minimize the time and amount of solvent used during your runs. This will help you achieve the most efficient runs possible.
To do this, you need to calculate the retention factor value of the compounds you are trying to separate. The solvent (or eluent) should be selected in such a way as to allow constituents to be separated effectively and is usually based on polarity.
If you are using a liquid chromatography column, you will usually check for separation on a TLC plate (thin layer chromatography) before making a run, to ensure that you have chosen the correct solvent or solvent ratios.
This phase is also called an adsorbent and is a solid. This phase is packed into the inside of your column. It is usually made of very fine particles, which can have various properties such as pores or holes.
These unique properties allow molecules of a certain size to either get trapped in the cavities of the beads or to simply pass by if they are too large.
This process will separate out molecules by their respective sizes, in a process called size-exclusion chromatography.
There are numerous adsorbent materials, which either retain molecules or let them pass through. As you might imagine, the key differences here are particle size and the stationary phase.
You should also take care to select your stationary phase and mobile phases correctly, so your applications run as intended.
We strongly advise that you conduct research to see which is the correct stationary phase for your specific application. For more detailed information about selecting the right options with it comes to column chromatography, we offer lab consulting services!
- This piece does not include a stopper for the 45/50 joint. You may purchase a Rodaviss stopper or our 2-Port Inlet Adapter separately.
- To support this column, we recommend our 100mm ring stand and chain clamps.
- The Chromatography column comes as a standalone piece of glass – only the PTFE stopcock, GL-18 cap and barbed fitting are included.
- Check out our guide on column chromatography here.